|
KMID : 1161420140170030350
|
|
Journal of Medicinal Food
2014 Volume.17 No. 3 p.350 ~ p.356
|
|
|
Schizonepeta tenuifolia Ethanol Extract Exerts Anti-Inflammatory Activity Through the Inhibition of TLR4 Signaling in Lipopolysaccharide-Stimulated Macrophage Cells
|
|
Byun Myung-Woo
|
|
|
Abstract
|
|
|
|
Inflammatory diseases remain the leading cause of mortality worldwide in both men and women. Schizonepeta tenuifolia (ST) exerts a wide range of physiological activities and has been found to possess beneficial efficacies against inflammation-related diseases; however, the molecular mechanisms underlying this anti-inflammatory activity remain to be elucidated. We investigated the molecular basis for the downregulation of toll-like receptor 4 (TLR4) signal transduction by ST ethanol extract in lipopolysaccharide (LPS)-stimulated macrophages. In this study, ST ethanol extract (100 μg/mL) did not induce cell cytotoxicity and was used in all the following experiments. Treatment of LPS-stimulated macrophages with ST ethanol extract resulted in a significant decrease in cyclooxygenase-2 and prostaglandin E2 levels, and inducible nitric oxide synthase-mediated NO production. LPS-induced expression of cell surface molecules (CD80 and CD86) and production of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) were inhibited by ST ethanol extract. Further, we also found that the anti-inflammatory activities of ST ethanol extract was caused by inhibition of LPS-induced activation of mitogen-activated protein kinases, such as extracellular signal-regulated kinase 1/2 and p38, and the translocation of nuclear factor κB through TLR4 in macrophages. Thus, ST ethanol extract may possess novel and potent therapeutic efficacy for the treatment of inflammatory disease.
|
|
|
KEYWORD
|
|
|
cell surface molecules, cytokine, mitogen-activated protein kinases, nuclear factor κB, Schizonepeta tenuifolia, toll-like receptor
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|